李爱霞，张文芳，闫金莲
（甘肃农业大学食品科学与工程学院，甘肃兰州 730070）
摘要：本论文对4株分离自甘肃、宁夏产区葡萄酒中的乳杆菌进行鉴定并通过调整改良MRS培养基分析植物乳杆菌对酒精浓度、pH和SO2浓度的耐受性。结果表明：16S rDNA鉴定得到3株植物乳杆菌，在单一因素影响下，3株菌在pH 3.0条件下有良好生长，在16%vol酒精浓度可生长，110 mg/L SO2浓度下可生长。在综合因素影响下，3株菌在所设置的9组处理下都可生长，其中在酒精浓度10%vol，pH 3.8，SO2浓度45 mg/L条件下可良好生长，在酒精浓度14%vol，pH 3.2，SO2浓度45 mg/L条件下可生长。
关键词：植物乳杆菌；葡萄酒；耐受性
中图分类号：TS261.1 文献标识码：A 文章编号：1674-506X（2021）02-0091-0006
Identification and Tolerance Analysis of Lactobacillus plantarum of Wine
LI Ai-xia, ZHANG Wen-fang, YAN Jin-lian
（College of Food Science and Engineering, Gansu Agricultural Univercity, Lanzhou Gansu 730070, China）
Abstract：In this study, 4 Lactobacillus isolated from wine production area of Gansu and Ningxia province were identified. Then, Lactobacillus plantarum were used as the test strains to investigate the tolerance of pH value, alcohol and SO2 concentration in modified MRS medium. The 16S rDNA analysis showed that there were 3 Lactobacillus plantarum. Analysis of tolerance experiments showed that 3 Lactobacillus plantarum were able to grow in modified MRS medium under different conditions (pH 3.0 alcohol content 16% vol, SO2 concentration 110 mg/L). Under the compound conditions, all 3 strains could grow and proliferate well of pH 3.8, alcohol content 10%vol, SO2 concentration 45 mg/L. All 3 strains could be able to grow in the presence of pH 3.2, alcohol content 14%vol, SO2 concentration 45 mg/L.
Keywords：Lactobacillus plantarum; wine; tolerance
doi：10.3969/j.issn.1674-506X.2021.02-013
收稿日期：2020-11-20
基金项目：甘肃农业大学盛彤笙科技创新基金项目（GSAU-STS-1741）
作者简介：李爱霞（1989-），女，硕士，讲师。研究方向：酿酒微生物。
引文格式：李爱霞,张文芳,闫金莲.葡萄酒中植物乳杆菌的鉴定及其耐受性分析[J].食品与发酵科技,2021,57(2):91-96.
